Yuksel Agca

Yuksel Agca
Assistant Professor
Reproductive biology and Cryobiology

DVM, Ankara University MS, University of Wisconsin-Madison

PhD, Purdue University-West Lafayette

Post-doctoral fellow, Indiana University School of Medicine

AgcaY@missouri.edu
Research emphasis: Dr. Agca's research interests are in the development of more efficient methods for cryopreservation of mammalian germplasm (i.e gametes, embryos, ovarian tissue) from scientifically and economically valuable (e.g. transgenics, knockouts) animal models for genome resource banking purposes. He is also interested in the development of assisted reproductive technologies in order to utilize such cryopreserved biomaterials more effectively.

Selected publications:
Agca C, Fritz J, Walker LC, Levey A, Chan A, Lah JJ, Agca Y (2008). Transgenic rats producing amyloid protein precursor as Alzheimer’s disease model; Transgene and internal APP are regulated similarly. BMC Neuroscience 2008, 9:28 doi:10.1186/1471-2202-9-28.
Varisli O, Uguz C, Agca C, Agca Y (2008). Motility and acrosomal integrity comparisons between electro-ejaculated and epididymal ram sperm after exposure to a range of anisosmotic solutions, cryoprotective agents and low temperatures. Anim Reprod Sci. doi:10.1016/j.anireprosci.2008.01.012.

Baska KM, Manandhar G, Feng D, Agca Y, Tengowski M, Sutovsky M, Sutovsky P (2007). Presence and activity of the extracellular ubiquitin system in the mammalian epididymal fluid. J Cell Physiol 215:684-696.

Van den Abbeel E, Schneider U, Liu J, Agca Y, Critser JK and Van Steirteghem A (2007). Osmotic responses and tolerance limits of human in-vitro matured MII oocytes to changes in external osmolalities and oolemma permeability characteristics. Hum Reprod. 22: 1959-1972.
S-H Yang, Agca Y, P-H Cheng, J-J Yang, C Agca and AWS Chan (2007). Enhanced transgenesis by intracytoplasmic injection of bald lentivirus. Genesis 45:177-183

Seo D, Agca Y., Critser JK and Feng ZC (2007). Development of sorting, aligning, and orienting bull, mouse, and human motile sperm using inexpensive micro-fluidic device operated by hydrostatic pressure. Microfluidics and Nanofluidics.3: 561-570.

Agca Y., Bauer B, Johnson D, Critser JK and Riley L. (2007). Detection of mouse parvovirus in Mus Musculus gametes, embryos and ovarian tissues by polymerase chain reaction assay. Comparative Medicine. 57: 10-15.

Bryda EC, Pearson M, Agca Y, Bauer B (2006). Method for detection and identification of multiple chromosomal integration sites in transgenic animals created with lentivirus,”. Biotechniques. 41: 715-719.
Mingxuan F, Agca Y, Critser JK and Feng ZC. (2006). Vibration study of the Piezo-driven pipettes immersed in viscous liquids. J. Applied Physics. 100 (7): Art. No. 074701.

Men H, Agca Y., Riley L., Critser, JK. (2006). Improved survival of vitrified porcine embryos after partial delipation through chemically stimulated lipolysis and inhibition of apoptosis. Theriogenology, 66: 2008-2016.

Spindler RE, Crichton B, Agca Y, Loskutoff, Critser JK, Gardner DK. and Wildt DE (2006). Improved felid embryo development by group culture is modified but not lost with hetero specific companions. Theriogenology 66:82-92.

Men H, Agca Y, Critser ES, Critser JK. (2005). Beneficial effects of serum supplementation during in vitro production of porcine embryos on their ability to survive cryopreservation. Theriogenology, 64:1340-1349.
Men H, Agca Y, Mullen SF., Critser ES., and Critser JK (2005). Osmotic tolerance of in vitro produced porcine blastocysts assessed by their morphological integrity and cellular actin filament organization. Cryobiology, 51:119-129.

Agca Y, Mullen SF, Liu J, Johnson-Ward J, Gould K, Chan A, Critser JK (2005). Osmotic Tolerance and Membrane Permeability Characteristics of Rhesus Monkey (Macaca mulatta) Spermatozoa. Cryobiology, 51: 1-14.
Agca Y and Critser JK (2005). Assisted Reproductive Technologies and Genetic Engineering in Rats. The Laboratory Rats, second edition Suckow MA, Weisbroth SH and Franklin CL, Academic Press, pp 165-190.

Agca Y, Mullen SF, Liu J, Johnson-Ward J, Gould K, Chan A, Critser JK (2005). Osmotic properties of chimpanzee (Pan troglodytes) spermatozoa. J Andrology. 26:470-477.

Men H, Agca Y, Rutledge JJ, Critser JK. (2004). Apoptosis during the development of oocytes and preimplantation embryos. A.R.T and Science 4 (2): 5-7.

Walters EM, Men H, Agca Y, Mullen SF, Critser ES and Critser JK (2005). Osmotic tolerance of mouse spermatozoa from various genetic backgrounds: acrosome integrity, membrane integrity, and maintenance of motility. Cryobiology 50:193-205.

Mullen SF, Agca Y, Critser J.K. (2004). Modeling the probability of MII spindle disruption in bovine oocytes as a function of total osmolality using logistic regression, and it is application toward improved CPA addition and removal procedures. Cell Preservation Tech. 2: 145-155.

Mullen SF, Agca Y, Broermann DC, Jenkins CL., Johnson CA, Critser J.K (2004). The effect of osmotic stress on the meiotic spindle of mature human oocytes, and the relevance to cryopreservation. Hum Reprod 19:1148-54.

Agca Y, Gilmore J, Byers M, Woods E J, Liu J and Critser JK (2002). Osmotic characteristics of mouse spermatozoa in the presence of extenders and sugars Biol Reprod 2002;67:1493-1501.
Critser JK, Agca Y, Woods E. Cryopreservation of immature and mature gametes. In Christopher J De Jonge and Christopher LR Barratt (Eds): “Assisted Reproductive Technology: Accomplishment and New Horizons” Cambridge University Press. Pp. 144-166.

Agca Y, and Critser JK (2002). Cryopreservation of spermatozoa in assisted reproduction. Seminars in Reproductive Medicine. 20:15-23.

Keskintepe L, Agca Y, Gabriella, Pacholczyk A., Machnicki A, Critser JK (2001). Use of Cryopreserved One-cell Embryos for the Production of Transgenic (Tg) Mice. Biol Reprod 65:407-411.

Agca Y. Cryopreservation of oocytes and ovarian tissue (2000). The National Academies, Institute for Laboratory Animal Research Journal 41:207-220.

Pfaff RT, Agca Y, Liu J, Woods E, Peter AT, Critser JK (2000). Cryobiology of Rat Embryos I: Determination of Zygote Membrane Permeability Coefficients for Water and Cryoprotectants, Their Activation Energies, and the Development of Improved Cryopreservation Methods. Biol Reprod 63:1294-1302.

Liu J, Woods E, Agca Y, Critser ES, Critser JK (2000). Cryobiology of Rat Embryos II: A Theoretical Model for the Development of Interrupted Slow Freezing Procedures. Biol Reprod 63:1303-1312.

Agca Y, Liu J, Critser ES, Critser JK (2000). The fundamental cryobiology of rat immature and mature oocytes: Hydraulic conductivity in the presence of Me₂SO, Me₂SO permeability, and their activation energies. J Exp Zool 286:523-533.

Agca Y, Rutledge JJ, Liu J, Critser ES, Critser JK (2000). The Effect of osmotic stress on the developmental competence of germinal vesicle and metaphase II stage bovine cumulus oocyte complexes and its relevance to cryopreservation Mol Reprod Dev 55:212-219.

Woods J, Liu J, Agca Y, Gilmore J, Critser JK (1999). Murine spermatozoa cryopreservation: Development of a defined cryoprotetcant medium Cryobiology 39: 284-285.

Agca Y, Liu J, McGrath JJ, Critser ES, Critser JK (1999) Temperature-dependent osmotic behavior of germinal vesicle- and metaphase II stage bovine oocytes in the presence of DMSO in relationship to cryobiology. Mol Reprod Dev 53: 59-67.

Agca Y, Monson RL, Northey DL, Peschel DE, Schaefer DM, Rutledge JJ (1998). Normal calves from transfer of biopsied, sexed and vitrified IVP bovine embryos. Theriogenology 50: 129-145.

Agca Y, Monson RL, Northey DL, Mazni OA, Schaefer DM, Rutledge JJ (1998). Transfer of fresh and cryopreserved IVP bovine embryos: Normal calving, birth weight and gestation lengths Theriogenology 50: 147-162.

Agca Y, Liu J, McGrath JJ, Peter AT, Critser ES, Critser JK (1998). Membrane permeability characteristics of metaphase II mouse oocytes at various temperatures in the presence of Me₂SO. Cryobiology 36: 287-300.

Agca Y, Liu J, Peter AT, Critser ES, Critser JK (1998). Effect of developmental stage on bovine oocyte plasma membrane water and cryoprotectant permeability characteristics. Mol Reprod Dev 49: 408-415.

Critser JK, Agca Y, Gunasena KT (1997). The cryobiology of mammalian oocytes. In Karow A and Critser JK (eds): "Reproductive Tissue Banking: Scientific principles" Academic Press. pp. 329-357.